Ultrasonic Cell Disruption Separation And Purification Equipment 40Khz

Ultrasonic Cell Disruption Separation And Purification Equipment 40Khz

Description:

40Khz ultrasonic cell fragmentation technology utilizes high-frequency ultrasound to generate cavitation effects, i.e. bubbles formed and collapsed in the liquid, which generate shear forces and shock waves on the surface of the cell membrane, ultimately leading to cell membrane rupture. The ultrasonic cell breaker is used for various types of cells, including bacteria, yeast, animal cells, plant cells, etc. It can effectively destroy cell walls and membranes, and obtain relatively pure intracellular substances.

The control of parameters such as ultrasonic frequency, power, and action time can control the crushing effect.

Parameters:

Application :

1. Extract intracellular enzymes, proteins, nucleic acids and other biological macromolecules
2. Preparation of bacterial lysates for vaccine production
3. Separate intracellular organelles such as mitochondria, lysosomes, etc
4. Preparation of cell membrane layer for biofilm research

The frequency of ultrasound does have a certain impact on the fragmentation effect of plant cells. The specific manifestations are as follows:

1. Frequency selection:
In general, it is more appropriate to use ultrasonic frequencies of 20-50 kHz for plant cells.
Low frequency (such as<20 kHz) can easily cause organelle damage, while high frequency (such as>100 kHz) has poor fragmentation effect.
2. The relationship between frequency and power:
High frequency ultrasound usually requires lower power to produce sufficient crushing effect.
Lower frequency ultrasound requires higher power to achieve the same crushing effect.
3. Frequency and selectivity:
Different frequencies of ultrasound may be more conducive to damaging specific cellular structures.
Lower frequencies are more conducive to damaging cell walls, while higher frequencies are more conducive to damaging cell membranes.
4. Frequency and temperature rise:
Higher frequency ultrasound can more effectively avoid excessive heating of the sample.
However, lower frequency ultrasound usually generates a significant temperature rise and requires cooling measures.

In summary, it is important to choose a reasonable frequency when using ultrasound to break plant cells. It is generally recommended to choose from the range of 20-50 kHz and optimize frequency and power parameters based on specific target cells.

Ultrasonic Cell Disruption Separation And Purification Equipment 40Khz